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Human Adenine Phosphoribosyltransferase (APRT) Protein
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Catalogue No: abx651292
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US$942.50
(Size: 50 µg)
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Datasheet SDS
Human Adenine Phosphoribosyltransferase (APRT) Protein is an active Human protein. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested. Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP.
Research Articles on Adenine Phosphoribosyltransferase (APRT)
| Target | Adenine Phosphoribosyltransferase (APRT) |
| Research Area | Enzymes and Kinases, Metabolic Pathways, Kidney Biomarkers |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Exhibits binding activity. Enzymatic activity has not been tested. |
| Biological Activity | A binding ELISA assay was carried out to detect the interaction of recombinant Human APRT and recombinant Human VCAM1. Briefly, APRT was diluted serially in PBS with 0.01% BSA, pH 7.4. 100 μl duplicates were then transferred to VCAM1-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with an APRT antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37°C. 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of of APRT and VCAM1 is shown in the figures. A dose-dependent effect was observed. |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P07741 (UniProt, ExPASy) |
| UniProt Secondary AC | G5E9J2, Q3KP55, Q68DF9 |
| UniProt Entry Name | APT_HUMAN |
| KEGG | hsa:353 |
| String | 9606.ENSP00000367615 |
| Molecular Weight | Calculated MW: 23.3 kDa Observed MW (SDS-PAGE): 23 kDa |
| Sequence Fragment | Met1-Glu180 |
| Sequence | MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 2000 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |