African Swine Fever Virus Envelope Protein p54 (ASFV p54) Protein

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Catalogue No: abx620017
Price: US$609.00
(Size: 100 µg)

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A: SDS-PAGE analysis of expression tests of the target protein using coomassie blue staining; B: WB analysis of the target protein using His-tag antibody. The negative control is marked Ø. Briefly, the bacterial cells were collected by centrifugation. The supernatant (medium) was lysed in a buffer containing PBS, pH 7.5. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer (8 M Urea). After centrifugation, the supernatant was collected (denatured protein extraction - DPE). The NPE and DPE fractions were analyzed by SDS-PAGE. The target genes were transfected in HEK293 cells, 1.5 ml of culture medium and cells were collected on 6th day of post-transfection. After semi-purification, the optimal expression was observed in cultutre medium, NPE and DPE fraction.
African Swine Fever Virus Envelope Protein p54 (ASFV p54) Protein African Swine Fever Virus Envelope Protein p54 (ASFV p54) Protein

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Datasheet SDS
African Swine Fever Virus Envelope Protein p54 (ASFV p54) Protein is a recombinant protein produced in Mammalian cells.

Target African Swine Fever Virus Envelope Protein p54 (ASFV p54)
Research Area Veterinary / Animal Health
Expression Recombinant
Tested Applications ELISA, WB, SDS-PAGE
Host Mammalian Cells
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Form Lyophilized
Activity Not tested
Purity > 90% (SDS-PAGE)
Reconstitution Reconstitute in sterile water to produce a stock solution.
Storage Aliquot and store at -20°C to 80°C. Avoid repeated freeze-thaw cycles.
Shelf Life 12 months.
UniProt Primary AC B2MUM6 (UniProt, ExPASy)
UniProt Entry Name B2MUM6_ASF
Gene Symbol P54
Molecular Weight Calculated MW: 16.87 kDa
Sequence Fragment Ser53-Leu184
Tag C-terminal His tag
Buffer Prior to lyophilization: PBS pH 7.4, containing 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Availability Shipped within 1-2 months.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on African Swine Fever Virus Envelope Protein p54 (ASFV p54)


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