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RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody
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Catalogue No: abx012524
Price:
US$50.75
(Size: 10 µg)
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Rabbit polyclonal antibody against Akt protein. Immunogen region is C-terminal. Specificity is as follows for the reactive species: H:S473/S474/S472, M:S473/S474/S472, R:S473/S474/S472.
Research Articles on RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473)
| Target | RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) |
| Clonality | Polyclonal |
| Target Modification | Ser473 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/3000, IHC: 1/50 - 1/100, ELISA: 1/5000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Serine473 (Q-F-SP-Y-S). |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P31749 (UniProt, ExPASy) P31751 (UniProt, ExPASy) Q9Y243 (UniProt, ExPASy) |
| Enzyme Commission Number | EC 2.7.11.1 |
| Sequence | CPHFPQFSYSASST |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Specificity | Detects endogenous levels of Akt only when phosphorylated at Serine 473. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |