AKT Serine/Threonine Kinase (AKT) Antibody

AKT Serine/Threonine Kinase (AKT) Antibody is a Rabbit Polyclonal antibody for the detection of Pan-AKT.

Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.

Target	AKT Serine/Threonine Kinase (AKT)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	ELISA, WB, IHC, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/200 - 1/800, IHC: 1/20 - 1/100, IF/ICC: 1/20 - 1/100, IP: 1/20 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	human AKT1/AKT2/AKT3
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	P31749 (UniProt, ExPASy) P31751 (UniProt, ExPASy) Q9Y243 (UniProt, ExPASy)
UniProt Secondary AC	B2RAM5, B7Z5R1, Q9BWB6
UniProt Entry Name	AKT1_HUMAN, AKT2_HUMAN, AKT3_HUMAN
Gene Symbol	AKT1, AKT2, AKT3
GeneID	207 208 10000
OMIM	164730 164731 611223
Molecular Weight	Observed MW: 60 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on AKT Serine/Threonine Kinase (AKT)

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