RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser129 (AKT1 pS129) Antibody

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Target	RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser129 (AKT1 pS129)
Clonality	Polyclonal
Target Modification	Ser129
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, DB
Host	Rabbit
Recommended dilutions	DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S129 of human AKT1.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P31749 (UniProt, ExPASy)
UniProt Secondary AC	B2RAM5, B7Z5R1, Q9BWB6
UniProt Entry Name	AKT1_HUMAN
NCBI Accession	NP_001014431.1 NP_001014432.1 NP_005154.2
KEGG	hsa:207
String	9606.ENSP00000451828
Molecular Weight	Calculated MW: 55.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse and Rat AKT1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser129 (AKT1 pS129)

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