AKT1 / 2 / 3 (pY315 / 316 / 312) Antibody

AKT1/2/3 (pY315/316/312) Antibody is a Rabbit Polyclonal antibody against AKT1/2/3 (pY315/316/312). The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Target	AKT1/2/3 (pY315/316/312)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/100 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic phosphorylated peptide around Y315 of human AKT1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P31749 (UniProt, ExPASy) P31751 (UniProt, ExPASy) Q9Y243 (UniProt, ExPASy)
Gene Symbol	AKT1, AKT2, AKT3
GeneID	207/208/10000
NCBI Accession	NP_001014431.1
Molecular Weight	Calculated MW: 48/55/51/54 kDa Observed MW: 56 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on AKT1/2/3 (pY315/316/312)

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