Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody

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Catalogue No: abx227137
Price: US$319.00
(Size: 40 µl)

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IHC-P analysis of expression of CD21 in follicular dendritic cells in the lymphoid follicle of the tonsil (4 µm section).
Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody

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Datasheet SDS
Alpha-(1,3)-Fucosyltransferase 4 (FUT4) Antibody is a Rabbit Monoclonal antibody for the detection of CD21.

Target Alpha-(1,3)-Fucosyltransferase 4 (FUT4)
Research Area Enzymes and Kinases
Clonality Monoclonal
Clone I472
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the C-terminal region of human CD21.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
Shelf Life Up to 24 months.
UniProt Primary AC P22083 (UniProt, ExPASy)
UniProt Secondary AC B2RMS0
UniProt Entry Name FUT4_HUMAN
Gene Symbol FUT4
GeneID 2526
OMIM 104230
HGNC 4015
KEGG hsa:2526
Ensembl ENSG00000196371
String 9606.ENSP00000351602
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Mix 1.21 g Tris and 0.37 g EDTA and dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  3. Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  4. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  5. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  6. Rinse twice in distilled water, 5 minutes each.
  7. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  8. Wash twice in distilled water, 5 minutes each.
  9. Antigen retrieval: immerse the slide in either Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 9.0), and incubate in a water bath for 20-30 minutes at 96-98 °C.
  10. Remove the slide from the water bath and allow to stand at room temperature for 15 minutes in the buffer used in the previous step.
  11. Rinse twice in distilled water, 5 minutes each.
  12. Wash twice in Wash Buffer, 5 minutes each.
  13. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  14. Wash 3 times with Wash Buffer, 5 minutes each.
  15. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  16. Wash 3 times in Wash Buffer, 5 minutes each.
  17. Apply the DAB chromagen for 1-3 minutes.
  18. Wash twice in distilled water, 5 minutes each.
  19. Stain in hematoxylin for 5 minutes.
  20. Wash 3 times in distilled water, 2 minutes each.
  21. Mount the slide for observation.
Research Articles on Alpha-(1,3)-Fucosyltransferase 4 (FUT4)


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