Annexin V (Biotin)
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Catalogue No: abx092323
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US$333.50
(Size: 100 µg)
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Datasheet SDS
Annexin V is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The Annexin V affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (Annexin V binding to PS is Ca2+ dependent). The assay combines Annexin V staining of PS membrane events with the staining of the cell nucleus with PI or AAD-7 to distinguish living cells from dead cells. Annexin V apoptosis detection is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner (cytoplasmic-facing) leaflet of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy. This product contains Annexin V (Biotin) only and does not include 10X Binding Buffer, PI or 7-AAD reagents.
Research Articles on Annexin V
| Target | Annexin V |
| Research Area | Cell Death, Apoptosis |
| Reactivity | General (All species) |
| Tested Applications | FCM |
| Recommended dilutions | FCM: 1-2 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Biotin |
| Form | Lyophilized |
| Reconstitution | Reconstitute in sterile deionized water to a concentration of up to 1 mg/ml to make a stock solution. Ensure the product is fully dissolved prior to use. |
| Storage | Store at -20°C in the dark. |
| Shelf Life | 12 months. |
| Buffer | Does not contain preservatives. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Analyze as soon as possible after staining as prolonged periods can increase the number of apoptotic or necrotic cells. Avoid extended exposure of samples to direct light to prevent the fluorophores from quenching. |