Anti-Mycoplasma Treatment Reagent (200X)
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Price:
US$58.00
(Size: 1 ml)
Orders to United States ship from United States
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Anti-Mycoplasma Treatment Reagent (200X) is used to avoid mycoplasma contamination in cell culture, and is provided as a yellow-green solution. This product inhbibits DNA replication and protein synthesis of mycoplasmas.
- Effective removal of mycoplasma.
- No effect on cell proliferation or growth.
- Broad range of action.
- Treatment is a permanent cure for most cell types.
Target |
Anti-Mycoplasma Treatment Reagent |
Research Area |
Cell Culture |
Form |
Liquid |
Purification |
0.1 μm filtered. |
Storage |
Store at 2-8°C for up to 2 weeks or at -20°C for up to 2 years. Avoid repeated freeze/thaw cycles. Avoid exposure to light. |
Validity |
Long periods of exposure to light will affect the validity. This can cause the color to change from yellow-green to grey-green or dark brown. Do not use if a color change is observed. |
Buffer |
Not applicable. |
Endotoxin Level |
< 1 EU/ml |
Concentration |
200X Osmolality: 120-150 mOsm/kg |
Availability |
Shipped within 5-7 working days. |
Note |
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Directions for use |
- Dilute Anti-Mycoplasma Treatment Reagent (200X) in freshly prepared complete culture medium at a 1/200 dilution.
- Cells should be rinsed with PBS at the start of treatment and in between media changes.
- Treatment should be carried out daily for 3 consecutive days for a total of 6 treatments. It is recommended to check once a week for mycoplasma contamination.
- If cells are still contaminated after 3 weeks, increase the concentration of the Treatment Reagent to 1/100.
- Once the infection is cleared, it is recommended to test cultures every 2 months to prevent re-contamination.
- This product can be used as a preventative measure, particularly if cells are being cultured for long periods of time or if equipment is shared. It is recommended to add to complete culture medium at a 1/1000 dilution every 2-3 weeks. If culturing embyonic stem cells, the recommended dilution is 1/2000.
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