Anti-Mycoplasma Treatment Reagent (200X) is used to avoid mycoplasma contamination in cell culture, and is provided as a yellow-green solution. This product inhbibits DNA replication and protein synthesis of mycoplasmas.
Effective removal of mycoplasma.
No effect on cell proliferation or growth.
Broad range of action.
Treatment is a permanent cure for most cell types.
Target
Anti-Mycoplasma Treatment Reagent
Research Area
Cell Culture
Form
Liquid
Purification
0.1 µm Sterile Filtered.
Storage
Store at 2-8°C. Avoid exposure to light.
Shelf Life
Up to 24 months.
Buffer
Not applicable.
Endotoxin Level
< 1 EU/ml
Concentration
200X Osmolality: 120-150 mOsm/kg
Availability
Shipped within 5-7 working days.
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
Dilute Anti-Mycoplasma Treatment Reagent (200X) in freshly prepared complete culture medium at a 1/200 dilution.
Cells should be rinsed with PBS at the start of treatment and in between media changes.
Treatment should be carried out daily for 3 consecutive days for a total of 6 treatments. It is recommended to check once a week for mycoplasma contamination.
If cells are still contaminated after 3 weeks, increase the concentration of the Treatment Reagent to 1/100.
Once the infection is cleared, it is recommended to test cultures every 2 months to prevent re-contamination.
This product can be used as a preventative measure, particularly if cells are being cultured for long periods of time or if equipment is shared. It is recommended to add to complete culture medium at a 1/1000 dilution every 2-3 weeks. If culturing embyonic stem cells, the recommended dilution is 1/2000.
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