Antibody Pair Support Kit (Competitive Method)
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Price:
US$261.00
(Size: 10 × 96 tests)
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Antibody Pair Support Kit (Competitive Method) contains reagents for use with an antibody pair for a Competitive ELISA or CLIA.
Kit contents: - Coating Buffer: 1 vial, lyophilized powder
- Blocking Buffer: 1 vial, lyophilized powder
- Standard Diluent: 1 vial, lyophilized powder
- Biotin-Conjugated Competitor Diluent: 1 vial, lyophilized powder
- Streptavidin-HRP Diluent: 1 vial, lyophilized powder
- Wash Buffer: 2 vials, lyophilized powder
- Streptavidin-HRP: 1 vial, 1.2 ml
- TMB Substrate Solution: 2 vials, 48 ml each
- Stop Solution: 2 vials, 30 ml each
Reactivity |
General (All species) |
Tested Applications |
ELISA |
Recommended dilutions |
Streptavidin-HRP should be diluted 1/100 using Streptavidin-HRP Diluent. |
Reconstitution |
Reconstitute and prepare working solutions before carrying out the assay. All working solutions should be colourless. - Coating Buffer: Reconstitute in 220 ml distilled water.
- Blocking Buffer: Reconstitute in 240 ml distilled water.
- Standard Diluent: Reconstitute in 160 ml distilled water.
- Biotin-Conjugated Competitor Diluent: Reconstitute in 120 ml distilled water.
- Streptavidin-HRP Diluent: Reconstitute in 120 ml distilled water.
- Wash Buffer: Reconstitute 1 vial in 2000 ml distilled water.
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Storage |
Store Streptavidin-HRP at -20 °C. All other components can be stored at 4 °C for up to 6 months. Reconstituted and working (appropriately diluted) solutions should be used immediately but can be stored at 4 °C for up to one week. Avoid repeated freeze/thaw cycles. It is recommended that solutions are made fresh before the experiment. |
Buffer |
The Stop Solution contains 0.1% sulphuric acid. |
Availability |
Shipped within 5-7 working days. |
Note |
This product is for research use only. |
Directions for use |
Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. |
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