Antibody Pair Support Kit (Sandwich Method)

Catalogue No: abx098958
Price: £180.00
(Size: 10 × 96 tests)
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Antibody Pair Support Kit (Sandwich Method) contains reagents for use with an antibody pair for a Sandwich ELISA or CLIA.

Kit contents:
  • Coating Buffer: 1 vial, lyophilized powder
  • Blocking Buffer: 1 vial, lyophilized powder
  • Standard Diluent: 1 vial, lyophilized powder
  • Detection Antibody Diluent: 1 vial, lyophilized powder
  • Streptavidin-HRP Diluent: 1 vial, lyophilized powder
  • Wash Buffer: 2 vials, lyophilized powder
  • Streptavidin-HRP: 1 vial, 1.2 ml
  • TMB Substrate Solution: 2 vials, 48 ml each
  • Stop Solution: 2 vials, 30 ml each


  • Reactivity General (All species)
    Tested Applications ELISA
    Recommended dilutions Streptavidin-HRP should be diluted 1/100 using Streptavidin-HRP Diluent.
    Reconstitution Reconstitute and prepare working solutions before carrying out the assay. All working solutions should be colourless.
    • Coating Buffer: Reconstitute in 220 ml distilled water.
    • Blocking Buffer: Reconstitute in 240 ml distilled water.
    • Standard Diluent: Reconstitute in 160 ml distilled water.
    • Detection Antibody Diluent: Reconstitute in 120 ml distilled water.
    • Streptavidin-HRP Diluent: Reconstitute in 120 ml distilled water.
    • Wash Buffer: Reconstitute 1 vial in 2000 ml distilled water.
    Storage Store Streptavidin-HRP at -20 °C. All other components can be stored at 4 °C for up to 6 months. Reconstituted and working (appropriately diluted) solutions should be used immediately but can be stored at 4 °C for up to one week. Avoid repeated freeze/thaw cycles. It is recommended that solutions are made fresh before the experiment.
    Buffer The Stop Solution contains 0.1% sulphuric acid.
    Directions for use Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use.
    Availability Shipped within 5-7 working days.
    Note This product is for research use only.
    Research Articles on Antibody Pair Support Kit (Sandwich Method)

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