Autophagy Protein 5 (APG5) Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Target	Autophagy Protein 5 (APG5)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500, IHC-P: 1/10 - 1/50, IF/ICC: 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide selected from the full length of human APG5.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9H1Y0 (UniProt, ExPASy)
UniProt Secondary AC	O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7
UniProt Entry Name	ATG5_HUMAN
NCBI Accession	NP_004840.1
String	9606.ENSP00000358072
Molecular Weight	Calculated MW: 32.4 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Autophagy Protein 5 (APG5)

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