Human Arginase-1 (ARG1) Protein (Active)
REQUEST MORE INFO
Catalogue No: abx651297
Price:
US$739.50
(Size: 50 µg)
Orders to United States ship from United States
(
Click on the image to see the image legend
Add to Cart
Buy It Now
GET A QUOTE
Documents
Datasheet SDS
Human Arginase-1 (ARG1) Protein (Active) is an active protein from Human.
Research Articles on Arginase-1 (ARG1)
| Target | Arginase-1 (ARG1) |
| Research Area | Enzymes and Kinases, Tumor Immunity, Infection Immunity, Hepatology |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | Arginase (ARG) is a member of the inducible manganese (Mn2+)-containing enzyme family that catalyse the final step of the urea cycle in the liver, converting l-arginine into l-ornithine and urea. To assess its biological activity, an enzymatic activity assay was performed. Briefly, recombinant human Arginase ARG was serially diluted in deionized water. Samples (40 μL) were added to microplate wells together with 10 μL of substrate mixture containing 100 mM L-arginine, 500 mM glycine, and 1.25 mM MnCl2 (pH 10.5). The reaction mixtures were incubated for 2 h at 37 °C. The enzymatic reaction was then terminated by the addition of 200 μL stop solution containing 2 mM o-phthalaldehyde (OPA), 2 mM N-(1-naphthyl)ethylenediamine (NED), 50 mM boric acid, 1 M sulfuric acid, and 0.03% Brij-35. Plates were covered and incubated for 20 min at room temperature, after which absorbance was measured at 520 nm in endpoint mode. One unit of enzyme activity was defined as the amount of enzyme required to produce 1 μmol of urea per minute. The specific activity of recombinant human ARG was determined to be > 24400 pmol/min/μg, as shown in Figure 3. |
| Purity | > 98% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P05089 (UniProt, ExPASy) |
| UniProt Secondary AC | A6NEA0, Q5JWT5, Q5JWT6, Q8TE72, Q9BS50 |
| UniProt Entry Name | ARGI1_HUMAN |
| Gene Symbol | ARG1 |
| GeneID | 383 |
| OMIM | 207800 |
| HGNC | 663 |
| Ensembl | ENSG00000118520 |
| Molecular Weight | Calculated MW: 36.9 kDa Observed MW: 37 kDa (SDS-PAGE) |
| Sequence Fragment | Met1-Lys322 |
| Sequence | MSAKSRTIGI IGAPFSKGQP RCGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPEADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLATGSISG HARVHPDLGV IWVDAHTDIN TPLTITSGNL HGQPVSFLLK ELKGKIPDVP GFSWTPCIIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEEILSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PK |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl and 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |