Rho GDP-Dissociation Inhibitor 1 (ARHGDIA) Antibody

REQUEST MORE INFO
Catalogue No: abx001126
Price: US$232.00
(Size: 20 µl)

Orders to United States ship from United States

Reviews: 0 Publications and Reviews
(0 Publications and Reviews)
Western blot analysis of lysates from wild type (WT) and RhoGDI knockout (KO) 293T cells, using [KO Validated] RhoGDI Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
Rho GDP-Dissociation Inhibitor 1 (ARHGDIA) Antibody Rho GDP-Dissociation Inhibitor 1 (ARHGDIA) Antibody

Click on the image to see the image legend

Available Options

* Size:



Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
ARHGDIA Antibody is a Rabbit Polyclonal antibody against ARHGDIA. Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4).

Target Rho GDP-Dissociation Inhibitor 1 (ARHGDIA)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant protein corresponding to ARHGDIA. The exact sequence is proprietary.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P52565 (UniProt, ExPASy)
UniProt Secondary AC A8MXW0, B2R5X1, B4DDD3, B4DUV9, Q6IBM5
UniProt Entry Name GDIR1_HUMAN
Gene Symbol ARHGDIA
GeneID 396
NCBI Accession NP_001172006.1
KEGG hsa:396
String 9606.ENSP00000269321
Molecular Weight Calculated MW: 23 kDa
Observed MW: 25 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Rho GDP-Dissociation Inhibitor 1 (ARHGDIA)


Write a review

Continue
Tags:
(Click to show)