Autophagy Related 4B Cysteine Peptidase (ATG4B) Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) conjugated form, and has the capacity for the binding to autophagosomes.

Target	Autophagy Related 4B Cysteine Peptidase (ATG4B)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.
Isotype	IgG
Form	Liquid
Purification	Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9Y4P1 (UniProt, ExPASy)
UniProt Entry Name	ATG4B_HUMAN
NCBI Accession	NP_037457.3 NP_847896.1
String	9606.ENSP00000384259
Molecular Weight	Calculated MW: 44.3 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse ATG4B.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Autophagy Related 4B Cysteine Peptidase (ATG4B)

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