Sodium/Potassium-Transporting ATPase Subunit Alpha 1 (ATP1A1) Antibody

ATP1A1 Antibody is a Rabbit Polyclonal antibody against ATP1A1. The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).

Target	Sodium/Potassium-Transporting ATPase Subunit Alpha 1 (ATP1A1)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	WB, IHC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P05023 (UniProt, ExPASy)
UniProt Entry Name	AT1A1_HUMAN
Gene Symbol	ATP1A1
GeneID	476
NCBI Accession	NP_000692.2
KEGG	hsa:476
String	9606.ENSP00000445306
Molecular Weight	Calculated MW: 113 kDa Observed MW: 113 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Sodium/Potassium-Transporting ATPase Subunit Alpha 1 (ATP1A1)

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