Beta Actin (ACTB) Antibody

Beta actin Antibody is a Mouse Monoclonal antibody against Bet actin. Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.

Target	Beta Actin (ACTB)
Clonality	Monoclonal
Clone	C344
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC, IF/ICC
Host	Mouse
Recommended dilutions	WB: 1/2000 - 1/20000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Fusion protein of human β-Actin
Isotype	IgG1 Kappa
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P60709 (UniProt, ExPASy)
UniProt Entry Name	ACTB_HUMAN
GeneID	60
KEGG	hsa:60
String	9606.ENSP00000349960
Molecular Weight	Calculated MW: 42 kDa Observed MW: 42 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	1.15 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Beta Actin (ACTB)

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