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Bid (pS65) Antibody
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Catalogue No: abx031834
Price:
US$326.25
(Size: 80 µl)
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Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8) ; CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Research Articles on Bid (pS65)
| Target | Bid (pS65) |
| Clonality | Polyclonal |
| Target Modification | Ser65 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P55957 (UniProt, ExPASy) |
| UniProt Secondary AC | Q549M7, Q71T04, Q7Z4M9, Q8IY86 |
| Molecular Weight | Calculated MW: 22 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
