Blocking Buffer and Coating Stabilizer

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Catalogue No: abx090811
Price: US$862.75
(Size: 1000 ml)

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Blocking Buffer and Coating Stabilizer

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Datasheet SDS
Blocking Buffer and Coating Stabilizer is a specially formulated reagent to improve the stability and function of antigens/proteins bound to a solid phase. Use as supplied for very unstable proteins or for less labile antigens/proteins dilute up to 1:1 in your current blocker.

Target Coating Stabilizer & Blocking Buffer
Conjugation Unconjugated
Form Liquid
Purification 0.2 µm filtered.
Storage Store at 2°C to 8°C. Do not freeze.
Shelf Life At least 12 months.
Buffer pH 7.2 ± 0.2. Contains a proprietary preservative (a non-mercury and azide free active ingredient against micro-organisms).
Specificity This product is a specially formulated reagent to improve the stability and function of antigens/proteins bound to a solid phase. Use as supplied for very unstable proteins or less labile antigens/proteins, dilute up to 1:1 in your current blocker.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Centrifuge before opening to ensure complete recovery of vial contents.

Recommended Protocol for Stabilizing and Blocking Immobilised Proteins:

  1. After coating the surface with protein/antigen, wash once to remove excess and weakly adsorbed protein.
  2. Before the protein begins to dry, coat the surface with the Coating Stabilizer and Blocking Buffer to completely cover the bound material. Allow to incubate at room temperature for 15 to 60 minutes.
  3. Aspirate or drain the excess stabilizer from the surface. Do not wash the surface.
  4. Dry the protein, preferably under vacuum. Recommended drying times are as follows:
    • Two hours under vacuum (< 100 micron).
    • Overnight in a humidity controlled chamber that registers < 15% humidity
  5. Package the bound antigen/protein in a sealed airtight container with desiccant. The product is now stabilized for long-term storage at 2–8 °C.
Research Articles on Coating Stabilizer & Blocking Buffer


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