Blood Genomic DNA Kit (with Magnetic Stand)

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Catalogue No: abx098867
Price: US$435.00
(Size: 50 rxns)

Orders to United States ship from United States

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Blood Genomic DNA Kit (with Magnetic Stand)

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Datasheet SDS
Blood Genomic DNA Kit (with Magnetic Stand) is an ideal solution for isolating high-quality DNA by specific adsorption of magnetic beads, with no centrifugation required.

Kit components:
  • Binding Buffer: 18 ml
  • Clean Buffer: 50 ml
  • Wash Buffer: 12 ml
  • Elution Buffer: 10 ml
  • Proteinase K (20 mg/ml): 1 ml
  • Magnetic Beads: 800 µl
  • Magnetic Stand (16 hole): 1


Target Blood Genomic DNA
Storage Store at room temperature.
Shelf Life 12 months.
Sample Type Whole blood.
Material Required But Not Provided
  • Pipettes and pipette tips
  • Microcentrifuge tubes
  • Ethanol (100%): 98 ml
  • Isopropanol (100%)
  • Vortex
Sample Collection/Preparation Whole blood: Use directly. Use within 1 week of collection if stored at 2-8 °C. For longer-term storage, store at -80 °C. Avoid repeated freeze-thaw cycles.
Reagent Preparation Bring all reagents to room temperature before use.
  • Clean Buffer Working Solution: Add 50 ml of 100% Ethanol to 50 ml Clean Buffer and mix fully.
  • Wash Buffer Working Solution: Add 48 ml of 100% Ethanol to 12 ml Wash Buffer and mix fully.
  • Magnetic Beads: Mix well with a vortex immediately before use.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Add 50-250 µl of whole blood to each 1.5 ml microcentrifuge tube.
  2. Add 300 µl of Binding Buffer and 20 µl of Proteinase K to the microcentrifuge tubes and mix well by vortexing.
  3. Stand at room temperature for 10 minutes, vortex 1-2 times during incubation.
  4. Add 450 µl Isopropanol to each microcentrifuge tubes and vortex for 10 seconds. Add 15 µl of well-mixed Magentic Beads into each microcentrifuge tubes.
  5. Vortex for 1 minute, then stand at room temperature for 3 minutes. Repeat for a total of four times.
  6. Place the microcentrifuge tubes on the magnetic stand. Gently turn the tubes left and right, then invert the stand 2-3 times. Stand at room temperature for 30 seconds. Remove as much supernatant as possible, being careful to not remove any beads.
  7. Remove the microcentrifuge tubes from the stand, add 800 µl of Clean Buffer Working Solution, vortex for 2 minutes, then place the tube back on the magnetic stand and remove the supernatant as described in step 6. Repeat for a total of two times.
  8. Remove the microcentrifuge tubes from the stand, add 500 µl of Wash Buffer Working Solution, vortex for 2 minutes, then place the tube back on the magnetic stand and remove the supernatant as described in step 6. Repeat for a total of two times.
  9. Uncap the tubes, and allow to air-dry for 10-15 minutes.
  10. Remove the tubes from the stand, add 100-200 µl of Elution Buffer to each tube, and mix gently by pipetting up and down several times to resuspend the beads. Incubate at 56 °C for 10 minutes, mixing gently by pipetting up and down 1-2 times during incubation.
  11. Place the tubes onto the stand, and separate beads as described in step 6. Carefully transfer the supernatant to a clean tube, and store the purified DNA at -20 °C

Note:

Sterile pipette tips must be used to avoid contamination with DNase.
Research Articles on Blood Genomic DNA


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