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Serine/threonine-Protein Kinase B-Raf (BRAF) Antibody
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Catalogue No: abx010442
Price:
US$427.75
(Size: 100 µl)
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B-Raf is a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.
Research Articles on Serine/threonine-Protein Kinase B-Raf (BRAF)
| Target | Serine/threonine-Protein Kinase B-Raf (BRAF) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, IHC |
| Host | Mouse |
| Recommended dilutions | ELISA: 1/10000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant fragment of BRAF expressed in E. coli. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Unpurified ascites. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P15056 (UniProt, ExPASy) |
| UniProt Entry Name | BRAF_HUMAN |
| Gene Symbol | BRAF |
| GeneID | 673 |
| OMIM | 114500 |
| HGNC | 1097 |
| KEGG | hsa:673 |
| Ensembl | ENSG00000157764 |
| String | 9606.ENSP00000288602 |
| Enzyme Commission Number | EC 2.7.1.37 EC 2.7.11.1 |
| Molecular Weight | 84.4 kDa |
| Buffer | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | Not determined. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |