Serine/threonine-Protein Kinase B-Raf (BRAF) Antibody

REQUEST MORE INFO
Catalogue No: abx002172
Price: US$217.50
(Size: 20 µl)

Orders to United States ship from United States

Serine/threonine-Protein Kinase B-Raf (BRAF) Antibody

Click on the image to see the image legend

Available Options

* Size:



Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
BRAF Antibody is a Rabbit Polyclonal antibody against BRAF. BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

Target Serine/threonine-Protein Kinase B-Raf (BRAF)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant protein corresponding to BRAF. The exact sequence is proprietary.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P15056 (UniProt, ExPASy)
UniProt Entry Name BRAF_HUMAN
Gene Symbol BRAF
GeneID 673
NCBI Accession NP_004324.2
KEGG hsa:673
String 9606.ENSP00000288602
Molecular Weight Calculated MW: 84 kDa
Observed MW: 87 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Serine/threonine-Protein Kinase B-Raf (BRAF)


Write a review

Tags:
(Click to show)