BRAF Antibody is a Rabbit Polyclonal antibody against BRAF. BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
| Target |
BRAF |
| Clonality |
Polyclonal |
| Reactivity |
Human, Mouse, Rat |
| Tested Applications |
WB |
| Host |
Rabbit |
| Recommended dilutions |
WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation |
Unconjugated |
| Immunogen |
A synthetic peptide of human BRAF. |
| Isotype |
IgG |
| Form |
Liquid |
| Purification |
Affinity purified. |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC |
P15056 (UniProt,
ExPASy)
|
| UniProt Entry Name |
BRAF_HUMAN |
| Gene Symbol |
BRAF |
| GeneID |
673
|
| KEGG |
hsa:673
|
| String |
9606.ENSP00000288602
|
| Molecular Weight |
Calculated MW: 84 kDa
Observed MW: 84 kDa |
| Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
| Concentration |
> 1 mg/ml |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |
Research Articles on Serine/threonine-Protein Kinase B-Raf (BRAF) Antibody
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