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Brain Derived Neurotrophic Factor (BDNF) Protein
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Catalogue No: abx650994
Price:
£370.00
(Size: 50 µg)
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BDNF Protein is a recombinant protein produced in E. coli using Prokaryotic expression. This protein is the immunogen for the following antibodies: abx132006
Research Articles on Brain Derived Neurotrophic Factor (BDNF)
| Target | Brain Derived Neurotrophic Factor (BDNF) |
| Origin | Human, Mouse, Rat, Pig, Horse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | Reconstitute to the original concentration in ddH2O. If further dilutions are required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0, to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Molecular Weight | Calculated MW: 27.4 kDa Observed MW (SDS-PAGE): 32 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Pro20-Arg252 |
| Sequence | P MKEANVRGQG SLAYPGVRTH GTLESVNGPK AGSRGLTSSS SSSLADTFEH VIEELLDEDQ KVRPNEENNK DADMYTSRVMLSSQVPLEPP LLFLLEEYKN YLDAANMSMR VRRHSDPARR GELSVCDSISEWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEGCRGIDKRHWN SQCRTTQSYV RALTMDSKKR IGWRFIRIDT SCVCTLTIKR GR |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
