Brain Derived Neurotrophic Factor (BDNF) Protein

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Catalogue No: abx650994
Price: US$536.50
(Size: 50 µg)

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Datasheet SDS
BDNF Protein is a recombinant protein produced in a Prokaryotic expression system (E. coli).

This protein is the immunogen for the following antibodies: abx132006

Target Brain Derived Neurotrophic Factor (BDNF)
Origin Human, Mouse, Rat, Pig, Horse
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Molecular Weight Calculated MW: 27.4 kDa
Observed MW (SDS-PAGE): 32 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Pro20-Arg252
Sequence P MKEANVRGQG SLAYPGVRTH GTLESVNGPK AGSRGLTSSS SSSLADTFEH VIEELLDEDQ KVRPNEENNK DADMYTSRVMLSSQVPLEPP LLFLLEEYKN YLDAANMSMR VRRHSDPARR GELSVCDSISEWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEGCRGIDKRHWN SQCRTTQSYV RALTMDSKKR IGWRFIRIDT SCVCTLTIKR GR
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Brain Derived Neurotrophic Factor (BDNF)


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