BrdU Antibody

Catalogue No: abx000064
Price: $364.00
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BrdU Antibody is a Mouse Monoclonal antibody against BrdU. The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

Target BrdU
Host Mouse
Clonality Monoclonal
Tested Applications IHC, IF/ICC, FCM
Recommended dilutions IHC: 1/50 - 1/500, IF/ICC: 1/50 - 1/500, FCM: 1/50 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Immunogen Synthetic Compound. Bromodeoxyuridine.
Purification Affinity purified.
Isotype IgG1
Conjugation Unconjugated
Storage Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Gene Symbol BrdU
Concentration > 1 mg/ml
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.

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