Bromodeoxyuridine (BrdU) Antibody

BrdU Antibody is a Mouse Monoclonal antibody against BrdU. The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

Target	Bromodeoxyuridine (BrdU)
Clonality	Monoclonal
Reactivity	General (All species)
Tested Applications	IF/ICC
Host	Mouse
Recommended dilutions	IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Bromodeoxyuridine
Isotype	IgG1 Kappa
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Gene Symbol	BRDU
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Bromodeoxyuridine (BrdU)

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