(Size: 20 µl)
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BrdU Antibody is a Mouse Monoclonal antibody against BrdU. The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
Research Articles on BrdU
||General (All species)
||IHC: 1/50 - 1/500, IF/ICC: 1/50 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
||Synthetic Compound. Bromodeoxyuridine.
||Purified by affinity chromatography.
||Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
||PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
||> 0.5 mg/ml
||Shipped within 5-10 working days.
||This product is for research use only.