Bromodeoxyuridine (BrdU) Antibody is a Mouse Monoclonal antibody against Bromodeoxyuridine (BrdU).

The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Measuring the uptake of BrdU is a reliable method to quantitatively measure the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

Target	Bromodeoxyuridine (BrdU)
Clonality	Monoclonal
Clone	A027
Reactivity	Human, Mouse, Rat
Tested Applications	IHC, IF/ICC
Host	Mouse
Recommended dilutions	IHC: 1/100 - 1/500, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH conjugated BrdU
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life	12 months.
Gene Symbol	BRDU
Buffer	PBS, pH 7.4, containing 0.05% stabilizer, 1% protein protectant, 50% glycerol.
Concentration	0.7 mg/ml
Availability	Shipped within 5-10 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Bromodeoxyuridine (BrdU)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue