C. trachomatis LGV Type-2 EB Protein

C. trachomatis LGV Type-2 (Strain 434) Elementary Bodies (EB) is a Protein isolated from Mouse L Cells. This product can be used for both IgG and IgM detection in assays, including enzyme immunoassays with polystyrene and latex solid phases and M capture formats.

Inactivation: Inactivated using gamma radiation. Effective primarily by damaging chlamydial genetic material. Effectiveness of inactivation is tested by inoculating an L cell monolayer with antigen. The culture is manipulated using the original optimal culture conditions used to manufacture the antigen. The culture is monitored for cytopathic effect for 5 days. If no signs of infection are observed the culture is passaged in to a fresh monolayer. The second passage is monitored for a further 5 day period. If no cytopathic effect is observed in either passage the antigen is considered inactivated. Result: No growth detected.

Target	C. trachomatis LGV Type-2 EB
Origin	Mouse
Tested Applications	ELISA
Host	Mouse
Conjugation	Unconjugated
Form	Liquid
Purification	Mouse L Cells were infected with C. trachomatis elementary bodies. Optimally infected cells were harvested and disrupted by sonication in PBS. Cellular debris was removed by centrifugation.
Storage	Store between -70 and -100 °C. Avoid repeated freeze/thaw cycles.
Buffer	PBS, pH 7.2. Buffer contains no preservative.
Concentration	4.5 mg/ml
Availability	Shipped within 10-20 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. This product is shipped with dry ice.
Directions for use	Centrifuge before opening to ensure complete recovery of vial contents. Sonicate immediately prior to use to ensure the preparation is uniform.

Research Articles on C. trachomatis LGV Type-2 EB

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