CD133 Antibody is a rabbit polyclonal antibody against human, mouse and rat CD133 (prominin-1). In humans, the CD133 glycoprotein is encoded by the PROM1 gene and is approximately 100-120 kDa in weight. This protein is a member of the pentaspan transmembrane glycoprotein (5-TM) family, which specifically localize to cellular protrusions. CD133 is expressed in a broad range of cell types including haematopoietic stem cells, mammary glands and cells of the digestive tract. Its expression is regulated by several different factors including TGF beta 1 and microRNA.
CD133 has also been identified as a key modulator of several different functions in cancer cells including tumorigenesis and metastasis which promotes their growth, spread and survival. As a result, CD133 is now frequently used as a biomarker for detecting and isolation stem cell and cancer cell populations. Abbexa's CD133 Antibody (abx019041) has been tested in several different applications including Western blotting (WB), immunohistochemistry, immunofluorescence (IF/ICC) and flow cytometry. This allows for the detection, observation and isolation of CD133 and CD133+ cells.
| Target |
CD133 |
| Reactivity |
Human, Mouse, Rat |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Tested Applications |
WB, IHC, IF/ICC |
| Recommended dilutions |
WB: 1/1000, IHC-P: 1/200, IF/ICC: 1/200, FCM: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
A synthetic peptide (conjugated with KLH) corresponding to near C-terminal of human CD133. |
| Purification |
Immunogen affinity purified. |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Swiss Prot |
O43490
|
| GeneID |
8842
|
| Gene Symbol |
PROM1 |
| OMIM |
604365
|
| HGNC |
9454 |
| Ensembl |
ENSG00000007062 |
| Buffer |
TBS, pH 7.4, 0.5% BSA, 40% glycerol and 0.05% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |
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