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| Target | T-Cell Surface Glycoprotein CD3 Zeta Chain Phospho-Tyr142 (CD247 pY142) |
| Research Area | Signal Transduction, Cellular Differentiation and Adhesion, Tumor Immunity, Infection Immunity, Cancer |
| Clonality | Polyclonal |
| Target Modification | Tyr142 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1/1000, WB: 1/500 - 1/3000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from Human T-Cell Surface Glycoprotein CD3 Zeta Chain (CD247) around the phosphorylation site of Tyrosine 142 (G-L-YP-Q-G). |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P20963 (UniProt, ExPASy) |
| UniProt Secondary AC | B1AK49, Q5VX13, Q8TAX4 |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Specificity | Detects endogenous levels of CD247 only when phosphorylated at Tyrosine 142. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |