Cyclin-Dependent Kinase 1 (CDK1) Antibody

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis.Cdc2 exists as a complex with both cyclin A and cyclin B.The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition.Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161.However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14.Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase.The isoform CDC2deltaT is found in breast cancer tissues.Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Target	Cyclin-Dependent Kinase 1 (CDK1)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB, IF/ICC, FCM
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IF/ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of CDC2 expressed in E. coli.
Isotype	IgG1
Form	Liquid
Purification	Unpurified ascites.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P06493 (UniProt, ExPASy)
UniProt Secondary AC	A8K7C4, C9J497, O60764
UniProt Entry Name	CDK1_HUMAN
Gene Symbol	CDK1
GeneID	983
OMIM	116940
HGNC	1722
KEGG	hsa:983
Ensembl	ENSG00000170312
String	9606.ENSP00000378699
Molecular Weight	34 kDa
Buffer	Ascitic fluid containing 0.03% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Cyclin-Dependent Kinase 1 (CDK1)

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