+44 (0) 1223 755950
+1 832 327 7413
Cyclin-Dependent Kinase Inhibitor 2A (CDKN2A) Antibody
REQUEST MORE INFO
Catalogue No: abx000647
Price:
US$232.00
(Size: 20 µl)
Orders to United States ship from United States
(![Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.](https://www.abbexa.com/image/cache/data/products/18B/abx000647_1-500x500.jpg "Cyclin-Dependent Kinase Inhibitor 2A (CDKN2A) Antibody")
Click on the image to see the image legend
Add to Cart
Buy It Now
GET A QUOTE
Documents
Datasheet SDS
CDKN2A Antibody is a Rabbit Polyclonal antibody against CDKN2A. This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Research Articles on Cyclin-Dependent Kinase Inhibitor 2A (CDKN2A)
| Target | Cyclin-Dependent Kinase Inhibitor 2A (CDKN2A) |
| Research Area | Tumor Immunity, Developmental Science, Cancer |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/2000 - 1/10000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P42771 (UniProt, ExPASy) |
| UniProt Entry Name | CDN2A_HUMAN |
| Gene Symbol | CDKN2A |
| GeneID | 1029 |
| OMIM | 600160 155601 |
| NCBI Accession | NP_000068.1 |
| HGNC | 1787 |
| KEGG | hsa:1029 |
| Ensembl | ENSG00000147889 |
| String | 9606.ENSP00000418915 |
| Molecular Weight | Calculated MW: 8/11/12/13/16/17 kDa Observed MW: 16 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |