Cell Cryopreservation Medium

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Catalogue No: abx298009
Price: US$261.00
(Size: 5 ml)

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Cell Cryopreservation Medium

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Datasheet SDS
Chemically-Defined Xeno-Free Cell Cryopreservation Medium is a ready-to-use animal-free medium that is DMSO-free and Protein-free. This product can be used improve the survival rate of cell types after cryopreservation, including mesenchymal stem cells and lymphocytes. Cryopreserved cells using this product can be stored directly in the freezer at -80°C.

Target Cell Cryopreservation Medium
Storage Store between 2-8°C.
Shelf Life 12 months.
Buffer The exact formulation is proprietary.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use It is recommended to perform a preliminary experiment without using the Cell Cryopreservation Medium to assess product performance. The product is provided in sterile packaging and should be used under sterile conditions.

Suggested Procedure - Cell Cryopreservation
  1. Collect suspension or adherent cells using typical methods. Ensure that the cells are growing well and that the survival rate is > 90% (e.g. cells are in the logarithmic growth phase). Centrifuge at 300 × g for 5 minutes, then discard the supernatant.
  2. Add Cell Cryopreservation Medium until the cell density is within 5 × 105 - 1 × 107 cells/ml. Mix slowly to form a cell suspension.
  3. Transfer the cell suspension to a cyrotube and store in a freezer at -80°C. If storage in liquid nitrogen is required, it is recommended to store overnight at -80°C before transferring the tube into liquid nitrogen. Ensure that the cryotube is completely sealed before storage.
Suggested Procedure - Cell Recovery
  1. Add 5-10 ml of complete medium prewarmed to 37°C into a centrifuge tube.
  2. Remove the cryotube containing the cells from the freezer or liquid nitrogen, and quickly place it in a 37°C water bath. Shake the cryotube gently until it is completely thawed.
  3. Transfer the cell suspension in the cryotube slowly (dropwise) into the medium. Mix gently. Centrifuge at 300 × g for 5 minutes, then discard the supernatant.
  4. Add preheated complete medium. Transfer to a culture vessel by pipetting gently. Incubate at 37°C, 5% CO2.
Research Articles on Cell Cryopreservation Medium


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