Chemiluminescence Immunoassay (CLIAs) are a variation of the standard ELISA detection method. In this assay, chemiluminescent labels, which produce light when excited by chemical energy, are used. It is a highly sensitive and specific method, with a dynamic range greater than that of immunoenzymatic technology,for the quantitative analysis of proteins. A further advantage to CLIA is that long incubation times are not required, which allows rapid analysis.
See below for a general protocol and recommendations.
- Capture Antibody
- Detection Antibody-HRP (Enzyme-Conjugated Reagent)
- Hydrogen Peroxide / Luminol (Chemiluminescent Substrate)
- Blocking Buffer
- PBS Wash Buffer
Note: The Capture and Detection Antibodies used must be against the target protein of present in the sample.
For more information, try our Chemiluminescence Immunoassay (CLIA) Troubleshooting Guide.