Cleaved LC3A Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Target	Cleaved LC3A
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50, IF/ICC: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.
Isotype	IgG
Form	Liquid
Purification	Peptide Affinity Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9H492 (UniProt, ExPASy)
UniProt Secondary AC	E1P5P4, E1P5P5, Q9BXW5
UniProt Entry Name	MLP3A_HUMAN
NCBI Accession	NP_115903.1 NP_852610.1
String	9606.ENSP00000363970
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat, Cow and Zebrafish Cleaved LC3A.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Cleaved LC3A

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