Collagenase IV

Collagenase IV is an enzyme purified from Clostridium histolyticum intended for cell and tissue disaggregation of islet tissues/cells (insulin receptor sites). Suitable for release of physiologically active rat hepatocytes.

Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro. This sequence is repeated frequently in collagen. Collagenase is unique among proteases in its ability to degrade the triplehelical native collagen fibrils commonly found in connective tissue. The collagenase most commonly used for tissue dissociation is a crude preparation containing clostripiopeptidase A and a number of other proteases, polysaccharidases, and lipases. This crude preparation is ideally suited for tissue dissociation because it contains the enzyme required to attack native collagen and reticular fibres, in addition to the enzymes which hydrolyze the other proteins, polysaccharides, and lipids in the extracellular matrix of connective and epithelial tissues. Crude collagenase can exhibit lot-to-lot variability and may produce occasional toxicity. The activity of these crude collagenase preparations has been correlated with their effectiveness at dissociating specific tissue types leading to the classification of crude collagenase preparations by type.

Target	Collagenase IV
Origin	Bacteria
Conjugation	Unconjugated
Form	Lyophilized
Reconstitution	Reconstitute in TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4) to a concentration of 1-2 mg/ml at 37 °C.
Storage	Store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	68-130 kDa
Buffer	Not applicable.
CAS Number	9001-12-1
Biological Activity	Collagen: ≥ 125 CDU/mg solid One Collagen Digestion Unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 µmol of leucine in 5 hours at pH 7.4, 37 °C in the presence of calcium ions. Furylacryloyl-Leu-Gly-Pro-Ala (FALGPA): 0.5-5.0 FALGPA U/mg solid One FALGPA Unit (U) hydrolyzes 1.0 µmol of FALGPA per minute at pH 7.5, 25 °C in the presence of calcium ions. Caseinase: ≥ 10 U/mg solid One Neutral Protease Unit (U) hydrolyzes casein to produce color equivalent to 1.0 µmol of tyrosine per 5 hours at pH 7.5, 37 °C. Clostripain: ≥ 0.1 U/mg solid One Clostripain Unit (u) hydrolyzes 1.0 µmol of BAEE per min at pH 7.6, 25°C in the presence of DTT.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Collagenase IV

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