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Conjugations

Fluorescence Spectraviewer - EmissionAF350AF405AF488AF532AF555AF5681/11400500600700800900020406080100120Emission Wavelength (nm)Relative Intensity (%)
AF350 DataAF350AF405AF488AF532AF555AF568AF594AF633AF647AF660AF680AF750AF790AMCAAPCAPC / C750APC / Cyanine 7ATTO 390ATTO 488ATTO 565ATTO 594ATTO 633ATTO 655ATTO 680ATTO 700CF-BlueCyanine 3Cyanine 5Cyanine 7DILDL 350DL 405DL 488DL 594DL 549DL 633DL 650DL 649DL 680DL 800FITCIF488IF555OC-515Pacific BluePEPE / ATTO 594PE / Cyanine 5PE / Cyanine 5.5PE / Cyanine 7PE / DL 594PerCPPerCP / Cyanine 5.5Texas RedTRITC
3801
3851
3861
3881
3901
4151
4161
4211001100
432100
442100
445100
450100
452100
4751
476100
4781
4901
4921
5001
5101
512100100
5151100100
5201001100
5251
52811
53011111
5351
5391
5401
5451
5461
5501
555100
56011
566100
567100
569100
5701
5741100
5751100
5761001100
57811
5841
590100
594100
600111
601100
6081
6101
6121100
6151
618100100
62010011
626100100
630111
6331
6341
6361
647100
65011111
6511001
65511
658100
660100
666100100
667100
6681
6701
673100100
6741
6751100
680100
6901
691100
693100
6951
698100
70011
7031001
70511
712100
71511
716100
7181
7191
72511
74511
7481
75011111
7601
7651
767100
7701
776100
782100
785100100
79411100
79511
7961
800111
805100
8371
8451
8461
8481
850111
8601
8611
8811
9051
9261
Fluorescence Spectraviewer - ExcitationAF350AF405AF488AF532AF555AF5681/11200300400500600700800900020406080100120Excitation Wavelength (nm)Relative Intensity (%)
AF350 DataAF350AF405AF488AF532AF555AF568AF594AF633AF647AF660AF680AF750AF790AMCAAPCAPC / C750APC / Cyanine 7ATTO 390ATTO 488ATTO 565ATTO 594ATTO 633ATTO 655ATTO 680ATTO 700CF-BlueCyanine 3Cyanine 5Cyanine 7DILDL 350DL 405DL 488DL 594DL 549DL 633DL 650DL 649DL 680DL 800FITCIF488IF555OC-515Pacific BluePEPE / ATTO 594PE / Cyanine 5PE / Cyanine 5.5PE / Cyanine 7PE / DL 594PerCPPerCP / Cyanine 5.5Texas RedTRITC
2151
2851
3001111
30511
3201
3221
3251
3401
346100
3501001
353100
390100
3941
40010011001111
401100
40511100
408100
4101
4181
4191
4201
4251
4281
43011
4351
4381
4461
4481
4541
4551
4601
4611
4681
4751
4801001001
4811
4851
4881
4901
493100100
495100
4981
499100
50011100111
5011
5041
5051
5071
5101
52011
5231
5251
53011
532100
540111
5441
55011
551100
552100
553100
555100
556100
562100
564100
565100100100100100100
5801001
59010011
5921
593100100
59511
5981
600111
6021
603100
6051
630100
632100
6351
638100
6411
64611
649100
6501
651100100100100
654100100
663100
666100
6701
6741
6781
679100
680111
681100
685111
692100
69511
70010011
7101
7151
7201
7291
743100
7451
7501001
7521
777100
785100
8001
8151
8321
8471
8691
8721

Lowest value = 200
Wavelength Range approximated from verified sources and scientific literatureMax. (100%) Excitation/Emission Wavelength data obtained from our labsTHRESHOLD: 5% intensity - bottom of range approximated from the lowest value (less than or equal to 5% intensity), and the top of range the highest value (less than or equal to 5% intensity)

Fluorescent Dyes

  • AF350
  • AF405
  • AF488
  • AF532
  • AF555
  • AF568
  • AF594
  • AF633
  • AF647
  • AF660
  • AF680
  • AF750
  • AF790
  • AMCA - Aminomethylcoumarin Acetate
  • APC - Allophycocyanin
  • APC/Cyanine 7
  • ATTO 390
  • ATTO 488
  • ATTO 565
  • ATTO 594
  • ATTO 633
  • ATTO 655
  • ATTO 680
  • ATTO 700
  • CF-Blue
  • Indocarbocyanine - Cyanine 3
  • Indodicarbocyanine - Cyanine 5
  • Cyanine 5.5
  • Cyanine 7
  • DIL - Di-indocarbocyanine
  • DL 350
  • DL 405
  • DL 488
  • DL 594
  • DL 633 
  • DL 650
  • DL 680
  • DL 800
  • DY634
  • FITC - Fluorescent Isothiocyanate
  • IF488 - iFluor 488
  • IF555 - iFluor 555
  • OC-515
  • Pacific Blue
  • PE - Phycoerythrin
  • PE/ATTO 594
  • PE/Cyanine 5
  • PE/Cyanine 5.5
  • PE/Cyanine 7
  • PE/DL 594
  • PerCP - Peridinin - Chlorophyll- Protein Complex
  • PerCP/Cyanine 5.5
  • Texas Red - Sulforhodamine 101 Acid Chloride
  • TRITC - Thetramethylrhodamine Isothiocyanate

Other

  • ALP - Alkaline phosphatase Conjugation
  • AP - Alkaline Phosphatase
  • BGAL - Beta-Galactosidase
  • BgG Bovine Gamma Globulin
  • Biotin
  • BSA - Bovine Serum Albumin
  • BTG - Bacterial Transglutaminase
  • GST - Glutathione-S-Transferase
  • HCY - Homocysteine
  • HRP - Horseradish Peroxidase
  • HSA - Human Serum Albumin
  • KLH - Keyhole Limpet Hemocyanin
  • OVA - Ovalbumin

 

NameColourExcitation Wavelength (nm)Emission Wavelength (nm)Molecular WeightQuantum Yield
AF555YELLOW5565741.250 kDa
Cyanine 3YELLOW5535660.767 kDaQY 0.12
DILYELLOW551569
DL 550YELLOW5625760.444 kDa
IF555YELLOW5555670.444 kDa
PEYELLOW480;565578240 kDa
TRITCYELLOW552576
AF594RED5906170.82 kDaQY 0.66
AF633RED6326471.2 kDa
AF647RED6506651.3 kDaQY 0.33
AF660RED6666911.1 kDa
AF680RED6797031.15 kDa
AF750RED7507761.3 kDa
AF790RED7858051.75 kDa
APCRED650660104 kDa
Cyanine 5RED6496660.792 kDaQY 0.28
Cyanine 5.5RED6756941.128 kDaQY 0.23
Cyanine 7RED7437670.818 kDaQY 0.28
DL 594RED593618
DL 633RED638658
DL 650RED654673
DL 680RED681698
DL 800RED777794
PerCPRED49067535 kDa
Texas RedRED5896150.625 kDa
AF488GREEN4955190.643 kDaQY 0.92
AF532GREEN5325550.724 kDaQY 0.61
AF555GREEN5555671.25 kDaQY 0.1
DL 488GREEN493518
FITCGREEN494520
IF488GREEN493512
AF350BLUE3464420.41 kDa
AF405BLUE4004211.028 kDa
AF568BLUE5806010.792 kDaQY 0.69
CF-BlueBLUE408450
DL 350BLUE353432
DL 450BLUE400420