Conjugations
Fluorescence Spectraviewer - Emission AF350 AF405 AF488 AF532 AF555 AF568 1/11 400 500 600 700 800 900 0 20 40 60 80 100 120 Emission Wavelength (nm) Relative Intensity (%) AF350 Data AF350 AF405 AF488 AF532 AF555 AF568 AF594 AF633 AF647 AF660 AF680 AF750 AF790 AMCA APC APC / C750 APC / Cyanine 7 ATTO 390 ATTO 488 ATTO 565 ATTO 594 ATTO 633 ATTO 655 ATTO 680 ATTO 700 CF-Blue Cyanine 3 Cyanine 5 Cyanine 7 DIL DL 350 DL 405 DL 488 DL 594 DL 549 DL 633 DL 650 DL 649 DL 680 DL 800 FITC IF488 IF555 OC-515 Pacific Blue PE PE / ATTO 594 PE / Cyanine 5 PE / Cyanine 5.5 PE / Cyanine 7 PE / DL 594 PerCP PerCP / Cyanine 5.5 Texas Red TRITC 380 1 385 1 386 1 388 1 390 1 415 1 416 1 421 100 1 100 432 100 442 100 445 100 450 100 452 100 475 1 476 100 478 1 490 1 492 1 500 1 510 1 512 100 100 515 1 100 100 520 100 1 100 525 1 528 1 1 530 1 1 1 1 1 535 1 539 1 540 1 545 1 546 1 550 1 555 100 560 1 1 566 100 567 100 569 100 570 1 574 1 100 575 1 100 576 100 1 100 578 1 1 584 1 590 100 594 100 600 1 1 1 601 100 608 1 610 1 612 1 100 615 1 618 100 100 620 100 1 1 626 100 100 630 1 1 1 633 1 634 1 636 1 647 100 650 1 1 1 1 1 651 100 1 655 1 1 658 100 660 100 666 100 100 667 100 668 1 670 1 673 100 100 674 1 675 1 100 680 100 690 1 691 100 693 100 695 1 698 100 700 1 1 703 100 1 705 1 1 712 100 715 1 1 716 100 718 1 719 1 725 1 1 745 1 1 748 1 750 1 1 1 1 1 760 1 765 1 767 100 770 1 776 100 782 100 785 100 100 794 1 1 100 795 1 1 796 1 800 1 1 1 805 100 837 1 845 1 846 1 848 1 850 1 1 1 860 1 861 1 881 1 905 1 926 1
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Fluorescence Spectraviewer - Excitation AF350 AF405 AF488 AF532 AF555 AF568 1/11 200 300 400 500 600 700 800 900 0 20 40 60 80 100 120 Excitation Wavelength (nm) Relative Intensity (%) AF350 Data AF350 AF405 AF488 AF532 AF555 AF568 AF594 AF633 AF647 AF660 AF680 AF750 AF790 AMCA APC APC / C750 APC / Cyanine 7 ATTO 390 ATTO 488 ATTO 565 ATTO 594 ATTO 633 ATTO 655 ATTO 680 ATTO 700 CF-Blue Cyanine 3 Cyanine 5 Cyanine 7 DIL DL 350 DL 405 DL 488 DL 594 DL 549 DL 633 DL 650 DL 649 DL 680 DL 800 FITC IF488 IF555 OC-515 Pacific Blue PE PE / ATTO 594 PE / Cyanine 5 PE / Cyanine 5.5 PE / Cyanine 7 PE / DL 594 PerCP PerCP / Cyanine 5.5 Texas Red TRITC 215 1 285 1 300 1 1 1 1 305 1 1 320 1 322 1 325 1 340 1 346 100 350 100 1 353 100 390 100 394 1 400 100 1 100 1 1 1 1 401 100 405 1 1 100 408 100 410 1 418 1 419 1 420 1 425 1 428 1 430 1 1 435 1 438 1 446 1 448 1 454 1 455 1 460 1 461 1 468 1 475 1 480 100 100 1 481 1 485 1 488 1 490 1 493 100 100 495 100 498 1 499 100 500 1 1 100 1 1 1 501 1 504 1 505 1 507 1 510 1 520 1 1 523 1 525 1 530 1 1 532 100 540 1 1 1 544 1 550 1 1 551 100 552 100 553 100 555 100 556 100 562 100 564 100 565 100 100 100 100 100 100 580 100 1 590 100 1 1 592 1 593 100 100 595 1 1 598 1 600 1 1 1 602 1 603 100 605 1 630 100 632 100 635 1 638 100 641 1 646 1 1 649 100 650 1 651 100 100 100 100 654 100 100 663 100 666 100 670 1 674 1 678 1 679 100 680 1 1 1 681 100 685 1 1 1 692 100 695 1 1 700 100 1 1 710 1 715 1 720 1 729 1 743 100 745 1 750 100 1 752 1 777 100 785 100 800 1 815 1 832 1 847 1 869 1 872 1
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Lowest value = 200
Fluorescent Dyes AF350 AF405 AF488 AF532 AF555 AF568 AF594 AF633 AF647 AF660 AF680 AF750 AF790 AMCA - Aminomethylcoumarin AcetateAPC - AllophycocyaninAPC/Cyanine 7 ATTO 390 ATTO 488 ATTO 565 ATTO 594 ATTO 633 ATTO 655 ATTO 680 ATTO 700 CF-Blue Indocarbocyanine - Cyanine 3 Indodicarbocyanine - Cyanine 5 Cyanine 5.5 Cyanine 7 DIL - Di-indocarbocyanineDL 350 DL 405 DL 488 DL 594 DL 633 DL 650 DL 680 DL 800 DY634 FITC - Fluorescent IsothiocyanateIF488 - iFluor 488IF555 - iFluor 555OC-515 Pacific Blue PE - PhycoerythrinPE/ATTO 594 PE/Cyanine 5 PE/Cyanine 5.5 PE/Cyanine 7 PE/DL 594 PerCP - Peridinin - Chlorophyll- Protein ComplexPerCP/Cyanine 5.5 Texas Red - Sulforhodamine 101 Acid ChlorideTRITC - Thetramethylrhodamine Isothiocyanate Other ALP - Alkaline phosphatase ConjugationAP - Alkaline PhosphataseBGAL - Beta-GalactosidaseBgG - Bovine Gamma Globulin Biotin BSA - Bovine Serum AlbuminBTG - Bacterial TransglutaminaseGST - Glutathione-S-TransferaseHCY - HomocysteineHRP - Horseradish PeroxidaseHSA - Human Serum AlbuminKLH - Keyhole Limpet HemocyaninOVA - Ovalbumin
Name Colour Excitation Wavelength (nm) Emission Wavelength (nm) Molecular Weight Quantum Yield AF555 YELLOW 556 574 1.250 kDa Cyanine 3 YELLOW 553 566 0.767 kDa QY 0.12 DIL YELLOW 551 569 DL 550 YELLOW 562 576 0.444 kDa IF555 YELLOW 555 567 0.444 kDa PE YELLOW 480;565 578 240 kDa TRITC YELLOW 552 576 AF594 RED 590 617 0.82 kDa QY 0.66 AF633 RED 632 647 1.2 kDa AF647 RED 650 665 1.3 kDa QY 0.33 AF660 RED 666 691 1.1 kDa AF680 RED 679 703 1.15 kDa AF750 RED 750 776 1.3 kDa AF790 RED 785 805 1.75 kDa APC RED 650 660 104 kDa Cyanine 5 RED 649 666 0.792 kDa QY 0.28 Cyanine 5.5 RED 675 694 1.128 kDa QY 0.23 Cyanine 7 RED 743 767 0.818 kDa QY 0.28 DL 594 RED 593 618 DL 633 RED 638 658 DL 650 RED 654 673 DL 680 RED 681 698 DL 800 RED 777 794 PerCP RED 490 675 35 kDa Texas Red RED 589 615 0.625 kDa AF488 GREEN 495 519 0.643 kDa QY 0.92 AF532 GREEN 532 555 0.724 kDa QY 0.61 AF555 GREEN 555 567 1.25 kDa QY 0.1 DL 488 GREEN 493 518 FITC GREEN 494 520 IF488 GREEN 493 512 AF350 BLUE 346 442 0.41 kDa AF405 BLUE 400 421 1.028 kDa AF568 BLUE 580 601 0.792 kDa QY 0.69 CF-Blue BLUE 408 450 DL 350 BLUE 353 432 DL 450 BLUE 400 420
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