Conjugations

Fluorescent Dyes

  • FITC - Fluorescent Isothiocyanate
  • AF350 - Alexa Fluor 350
  • AF488 - Alexa Fluor 488
  • AF555 - Alexa Fluor 555
  • AF594 - Alexa Fluor 594
  • AF647 - Alexa Fluor 647
  • PE - Phycoerythrin
  • APC - Allophycocyanin
  • Cy3 - Indocarbocyanine - Cyanine 3
  • Cy5 - Indodicarbocyanine - Cyanine 5
  • Cy7 - Cyanine 7
  • Cy5.5 - Cyanine 5.5
  • Texas Red - Sulforhodamine 101 acid chloride
  • PerCP Peridinin - Chlorophyll- Protein Complex
  • TRITC - Thetramethylrhodamine Isothiocyanate
  • RRX - Rhodamine Red X
  • DyLight 405
  • DyLight 488
  • DyLight 549
  • DyLight 594
  • DyLight 649
  • DyLight 680
  • DyLight 800

Other

  • BSA - Bovine Serum Albumin
  • BTG - Bacterial Transglutaminase
  • KLH - Keyhole Limpet Hemocyanin
  • OVA - Ovalbumin
  • HRP - Horseradish Peroxidas
  • AP - Alkaline Phosphatase
  • BGG - Bovine Gamma Globulin
  • ALP - Alkaline phosphatase Conjugation
  • AMCA – Aminomethylcoumarin
  • Agarose
  • Biotin

 

Name Colour Excitation Wavelength (nm) Emission Wavelength (nm) Molecular Weight Quantum Yield
Alexa Fluor 350 BLUE 346 442
FITC GREEN 494 520
Alexa Fluor 488 GREEN 495 519
TRITC YELLOW 547 572
Cy3 YELLOW (512);550 570;(615) 767 QY 0.12
Alexa Fluor 555 YELLOW 555 565
PE YELLOW 480;565 578 240 k
Cy5.5 RED 675 694 1128 QY 0.28
Cy7 RED 743 767 818 QY 0.28
Cy5 RED (625);650 670 792 QY 0.27
PerCP RED 490 675 35 kDa
APC RED 650 660
Alexa Fluor 594 RED 590 617
Alexa Fluor 647 RED 650 665
Texas Red RED 589 615 625