Gap Junction Alpha-5 Protein / CX40 (GJA5) Antibody

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

Target	Gap Junction Alpha-5 Protein / CX40 (GJA5)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/4000, IHC-P: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P36382 (UniProt, ExPASy)
UniProt Secondary AC	Q5T3B6, Q5U0N6
UniProt Entry Name	CXA5_HUMAN
NCBI Accession	NP_005257.2 NP_859054.1
KEGG	hsa:2702
String	9606.ENSP00000484552
Molecular Weight	Calculated MW: 40.4 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse GJA5.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Gap Junction Alpha-5 Protein / CX40 (GJA5)

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