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Target | Brain Derived Neurotrophic Factor (BDNF) |
Research Area | Cytokines, Infection Immunity, Neuroscience |
Origin | Cow |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Activity | Not tested |
Purity | > 90% |
Reconstitution | Reconstitute in ddH2O. The stock concentration should be between 0.1-0.2 mg/ml. Do not vortex. |
Storage | Store at 2-8°C for up to one month. For long-term storage, store at -80°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | Q95106 (UniProt, ExPASy) |
UniProt Entry Name | BDNF_BOVIN |
Molecular Weight | Calculated MW: 27.3 kDa Observed MW (SDS-PAGE): 32 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Pro20-Arg250 |
Sequence | P MKEANLRAQG SLAYPGVRTH GTLESMNGPK VGSRGLTSSS SLADTFEHVI EELLDEDQKV RPSEENNKDA DMYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVR RHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl and 5% Trehalose. |
Concentration | Prior to lyophilization: 200 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |