Transcriptional Repressor CTCF (CTCF) Antibody

CTCF Antibody is a Rabbit Polyclonal antibody against CTCF. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Target	Transcriptional Repressor CTCF (CTCF)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts, ChIP: 5 µg/10-15 µg Chromatin. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P49711 (UniProt, ExPASy)
UniProt Secondary AC	B5MC38, Q53XI7, Q59EL8
UniProt Entry Name	CTCF_HUMAN
Gene Symbol	CTCF
GeneID	10664
NCBI Accession	NP_006556.1
KEGG	hsa:10664
String	9606.ENSP00000264010
Molecular Weight	Calculated MW: 83 kDa Observed MW: 140 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Transcriptional Repressor CTCF (CTCF)

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