Cullin 1 (CUL1) Antibody

Core component of multiple cullin-RING-based SCF (SKP1-Cullin 1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF (BTRC) and SCF (FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF (FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF (BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF (SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF (SKP2) directs ubiquination of ORC1L, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF (FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF (FBXW2) directs ubiquitination of GCM1. SCF (FBXO32) directs ubiquitination of MYOD1. SCF (FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF (FBXO33) directs ubiquitination of YBX1. SCF (FBXO11) does not seem to direct ubiquitination of TP53. SCF (BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF (Cyclin F) directs ubiquitination of CP110 (By similarity).

Target	Cullin 1 (CUL1)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 680-708 amino acids from the C-terminal region of human CUL1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q13616 (UniProt, ExPASy)
UniProt Secondary AC	D3DWG3, O60719, Q08AL6, Q8IYW1
UniProt Entry Name	CUL1_HUMAN
KEGG	hsa:8454
String	9606.ENSP00000326804
Molecular Weight	Calculated MW: 89.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse CUL1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Cullin 1 (CUL1)

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