Human Cytochrome P450 1A2 (CYP1A2) Protein (Active)
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Catalogue No: abx651319
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(Size: 50 µg)
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Datasheet SDS
Human Cytochrome P450 1A2 (CYP1A2) Protein (Active) is an active protein from Human. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Cytochrome P450 1A2 (CYP1A2)
| Target | Cytochrome P450 1A2 (CYP1A2) |
| Research Area | Metabolism |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | Cytochrome P450 1A2 (CYP1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acid Ceramidase (ASAH1) has been identified as an interactor of CYP1A2 through affinity capture-MS, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ASAH1-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-CYP1A2 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of CYP1A2 and ASAH1 is shown in Figure 4. |
| Purity | > 95% |
| Reconstitution | Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P05177 (UniProt, ExPASy) |
| UniProt Secondary AC | Q16754, Q6NWU3, Q6NWU5, Q9BXX7, Q9UK49 |
| UniProt Entry Name | CP1A2_HUMAN |
| KEGG | hsa:1544 |
| String | 9606.ENSP00000342007 |
| Molecular Weight | Calculated MW: 55.2 kDa Observed MW: 55 kDa |
| Sequence Fragment | Ala2-Ser231 |
| Sequence | ALSQSVPFS ATELLLASAI FCLVFWVLKG LRPRVPKGLK SPPEPWGWPL LGHVLTLGKN PHLALSRMSQ RYGDVLQIRI GSTPVLVLSR LDTIRQALVR QGDDFKGRPD LYTSTLITDG QSLTFSTDSG PVWAARRRLA QNALNTFSIA SDPASSSSCY LEEHVSKEAK ALISRLQELM AGPGHFDPYN QVVVSVANVI GAMCFGQHFP ESSDEMLSLV KNTHEFVETA S |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |