Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein
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Catalogue No: abx261980
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US$261.00
(Size: 5 µg)
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Datasheet SDS
Human KRT18 Protein is a recombinant Human protein expressed in E. coli.
Research Articles on Keratin, Type I Cytoskeletal 18 (KRT18)
| Target | Keratin, Type I Cytoskeletal 18 (KRT18) |
| Research Area | Tumor Immunity, Pulmonology, Dermatology, Cancer |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 95% (SDS-PAGE) |
| Purification | Purified by proprietary chromatographic techniques. |
| Reconstitution | Reconstitute in sterile 18 MΩ · cm water to a concentration of at least 0.7 mg/ml. This stock solution can be further diluted if required. Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS. |
| Storage | Store lyophilized at -18°C for long term storage. It is recommended to add a carrier protein (0.1% HSA or BSA). Upon reconstitution, store at 4°C for up to 1 week. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P05783 (UniProt, ExPASy) |
| UniProt Secondary AC | Q53G38, Q5U0N8, Q9BW26 |
| UniProt Entry Name | K1C18_HUMAN |
| KEGG | hsa:3875 |
| String | 9606.ENSP00000373487 |
| Molecular Weight | Calculated MW: 48.2 kDa Observed MW (SDS-PAGE): 45 kDa |
| Buffer | Prior to lyophilization: 30 mM Tris-HCl, pH 8, containing 9.5 M urea, 2 mM EDTA, 2 mM DTT and 1 0mM methylammonium chloride. |
| Concentration | Prior to lyophilization: 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |