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|Quality Control||Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.|
|Storage||Shipped at 4 °C. Store at -20 °C for up to one year.|
|UniProt Primary AC||Q32ZH8 (UniProt, ExPASy)|
|Specificity||DEFB13 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.|
|Directions for use||1. Transfect with 100 nM siRNA 48 to 72 hours prior to cell lysis.
2. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.
3. Resuspend the siRNA oligos to an appropriate concentration with DEPC water.
Each vial is suitable for 250 transfections in a 24 well plate (20 pmol for each well).
|Availability||Shipped within 5-10 working days.|
|Note||This product is for research use only.|