Human Dihydrofolate Reductase (DHFR) Enzyme
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Catalogue No: abx073077
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US$261.00
(Size: 10 µg)
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Datasheet SDS
Human Dihydrofolate Reductase (DHFR) Enzyme is a recombinant enzyme consisting of a single, non-glycosylated polypeptide chain containing 207 amino acids, expressed in E. coli.
Research Articles on Dihydrofolate Reductase (DHFR)
| Target | Dihydrofolate Reductase (DHFR) |
| Research Area | Enzymes and Kinases |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
| Activity | Active |
| Biological Activity | Specific activity is >2000 pmol/min/µg is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at (pH 6.5, 25°C). |
| Purity | > 95% (SDS-PAGE) Sterile filtered. |
| Purification | Purified by proprietary chromatographic techniques. Sterile Filtered. |
| Storage | Store at 4°C for 2-4 weeks. Store at -20°C for long term storage. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P00374 (UniProt, ExPASy) |
| UniProt Secondary AC | B4DDD2, Q14130, Q6IRW8 |
| UniProt Entry Name | DYR_HUMAN |
| KEGG | hsa:1719 |
| String | 9606.ENSP00000396308 |
| Molecular Weight | 23.6 kDa |
| Sequence Fragment | 1-187 AA |
| Sequence | MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND. |
| Tag | N-terminal His tag |
| Buffer | 20 mM Tris-HCl, pH 8.0, containing 0.1 M NaCl, 2 mM DTT and 30% Glycerol. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |