DMEM Powder (High Glucose) with L-Glutamine, Sodium Pyruvate and Phenol Red

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting mammalian cell growth.

The formulation of this product does not contain L-Alanyl-L-Glutamine, HEPES or Pen/Strep.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X DMEM. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X DMEM.

Target	Dulbecco's Modified Eagle Medium (DMEM)
Research Area	Cell Culture
Form	Lyophilized
Purification	0.1 µm Sterile Filtered.
Reconstitution	For preparation of 1 L of DMEM, High Glucose: Dissolve 13.48 g or 1 pouch of DMEM, High Glucose (Powder) in ultrapure water. Add 2.2 g of NaHCO3 and add ultrapure water until it is fully dissolved. If required, adjust the pH with 1 M NaOH or 1 M HCl. Add ultrapure water to a final volume of 1 L. For cell culture applications, sterilize the solution using a 0.22 µm filter under aseptic conditions.
Storage	Store at 2-8°C. Avoid exposure to light.
Shelf Life	Up to 12 months.
Buffer	pH 5.8-6.2, containing 584 mg/L L-Glutamine, 4500 mg/L D-Glucose, 110 mg/L Sodium Pyruvate, 15 mg/L Phenol Red
Concentration	1X
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use	This product should be used with serum (or equivalent) supplementation. Use aseptic techniques to avoid contamination.

Research Articles on Dulbecco's Modified Eagle Medium (DMEM)

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