DMEM, High Glucose (Powder)

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Catalogue No: abx295009
Price: US$58.00
(Size: 1 × 10 L)

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Datasheet
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting mammalian cell growth. It contains a high concentration of glucose along with various amino acids, vitamins and inorganic salts essential for cell growth, though does not contain proteins, lipids or growth factors.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X DMEM. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X DMEM.

Target DMEM
Form Lyophilized
Reconstitution For preparation of 1 L of DMEM, High Glucose: Dissolve 13.48 g or 1 pouch of DMEM, High Glucose (Powder) in ultrapure water. Add 2.2 g of NaHCO3 and add ultrapure water until it is fully dissolved. If required, adjust the pH with 1 M NaOH or 1 M HCl. Add ultrapure water to a final volume of 1 L. For cell culture applications, sterilize the solution using a 0.22 µm filter under aseptic conditions.
Storage Store between 2-8 °C. Avoid exposure to light.
Validity Up to 12 months.
Buffer After reconstitution: pH 7.2-7.4, containing 4500 mg/L L-glucose, 4 mM L-glutamine, 15 mg/L phenol red and 1 mM sodium pyruvate. Does not contain HEPES or NaHCO3.
Concentration 1X
Availability Shipped within 5-7 working days.
Note This product is for research use only.
Directions for use This product should be used with serum (or equivalent) supplementation.
Use aseptic techniques to avoid contamination.
Research Articles on DMEM


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