DNA Staining Reagent

DNA Staining Reagent is a sensitive stain for detecting double-stranded DNA (dsDNA). It is provided as a 6X concentrated reagent for preparing DNA markers and samples for loading on agarose or polyacrylamide gels, and is a safe and non-hazardous alternative to ethidium bromide. It contains three tracking dyes (Orange G, Bromophenol Blue and Xylene Caynol FF) for visually tracking DNA migration during electrophoresis.

Approximate fluorescence excitation/emission maxima: 495/537 nm (when bound to nucleic acids).

Target	DNA Staining Reagent
Tested Applications	SDS-PAGE
Storage	Store at 4 °C for up to 12 months.
Buffer	Contains Orange G, Bromophenol Blue and Xylene Caynol FF.
Availability	Shipped within 10-15 working days.
Note	This product is for research use only.
Directions for use	Vortex for 10 seconds prior to use. For each 500 µl of DNA sample, add 100 µl of DNA Staining Reagent. Mix thoroughly. It is recommended that the DNA mass in the sample is greater than 50 ng, as this will not cause an obvious shift. DNA Staining Reagent should also be added to DNA markers to visualise the ladder bands. Load the sample and run accordingly. After electrophoresis, remove the gel and place a UV or visible-light transilluminator to visualise the bands. If required, gels can be post-stained with ethidium bromide.

Research Articles on DNA Staining Reagent

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