Dynamin 1 Like Protein (DNM1L) Antibody

DNM1L Antibody is a Rabbit Polyclonal antibody against DNM1L. Dynamin-related protein 1 (DNM1L) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DNM1L affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DNM1L clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DNM1L at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DNM1L stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DNM1L is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DNM1L is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6).

Target	Dynamin 1 Like Protein (DNM1L)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 411-710 of human DRP1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O00429 (UniProt, ExPASy)
UniProt Entry Name	DNM1L_HUMAN
Gene Symbol	DNM1L
GeneID	10059
NCBI Accession	NP_036193.2
KEGG	hsa:10059
String	9606.ENSP00000449089
Molecular Weight	Calculated MW: 82 kDa Observed MW: 78 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Dynamin 1 Like Protein (DNM1L)

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