Dog Cerebral Cortex Neuron Cells (CCNC)

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Catalogue No: abx700308
US$2,189.50 US$1,532.65
(Size: 5 × 10^5 cells)

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Dog Cerebral Cortex Neuron Cells (CCNC) Dog Cerebral Cortex Neuron Cells (CCNC) Dog Cerebral Cortex Neuron Cells (CCNC)

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Datasheet SDS
Dog Cerebral Cortex Neuron Cells (CCNC) are adherent Neuron Cells from Beagle Dog Cerebral cortex (aged 1-3 days). CCNC are cryopreserved at P0 and delivered frozen.

Target Cerebral Cortex Neuron Cells (CCNC)
Research Area Nervous Cells, Neurons, Cerebral and Nervous Systems
Origin Dog
Host Dog
Biological Activity Cell activity: > 85% (viability by Trypan Blue exclusion)
Purity > 85%
Negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Storage Shipped at -70°C. Upon receipt, store in liquid nitrogen (-196°C). Avoid repeated freeze/thaw cycles.
Shelf Life 12 months.
Buffer Contains 90% FBS and 10% DMSO.
Availability Shipped within 7-15 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

This product is shipped with dry ice.
Directions for use

Recommended Cell Culture Conditions:

Neurobasal-A Medium + B-27 Supplement (50X) + 1% Penicillin-Streptomycin Solution @ 37 °C, 95% air, 5% CO2.

Recommended Coating Conditions:

Poly-D-Lysine (0.1 mg/ml, 2 ml per T25 Flask).

Cell Recovery: Thaw cells in a 37 °C water bath with shaking until the mixture has dissolved. Transfer to a centrifuge tube and add culture medium (see Recommended Cell Culture Conditions above) at a volume 3-5 times the volume of the cells. Centrifuge at 1000 RPM for 5 minutes and discard the supernatant. Transfer to a precoated T25 flask for culture (see Recommended Coating Conditions above). It is recommended to change the medium every 2-3 days.

Cell Passage: CCNC cells do not proliferate in culture, therefore it is not recommended to use CCNC cells for expansion or long-term cultures.

Research Articles on Cerebral Cortex Neuron Cells (CCNC)


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