EGFR (pY1125) Antibody
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Catalogue No: abx031963
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US$326.25
(Size: 80 µl)
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Datasheet SDS
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Research Articles on EGFR (pY1125)
| Target | EGFR (pY1125) |
| Clonality | Polyclonal |
| Target Modification | Tyr1125 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, DB |
| Host | Rabbit |
| Recommended dilutions | DB: 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1125 of human EGFR. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at 2-8°C for up to 2 weeks, or at -20°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P00533 (UniProt, ExPASy) |
| UniProt Entry Name | EGFR_HUMAN |
| KEGG | hsa:1956 |
| String | 9606.ENSP00000275493 |
| Molecular Weight | Calculated MW: 134 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |