Alpha-Enolase (ENO1) Antibody

ENO1 Antibody is a Rabbit Polyclonal antibody against ENO1. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Target	Alpha-Enolase (ENO1)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	WB, IHC, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/1000 - 1/5000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P06733 (UniProt, ExPASy)
UniProt Secondary AC	B2RD59, P22712, Q16704, Q4TUS4, Q53FT9, Q53HR3, Q658M5, Q6GMP2, Q71V37, Q7Z3V6, Q8WU71, Q96GV1, Q9BT62, Q9UCH6, Q9UM55
UniProt Entry Name	ENOA_HUMAN
Gene Symbol	ENO1
GeneID	2023
OMIM	172430
NCBI Accession	NP_001188412.1 NM_001201483.1 NP_001419.1 NM_001428 NM_001428.3
HGNC	3350
KEGG	hsa:2023
Ensembl	ENSG00000074800
Molecular Weight	Calculated MW: 47 kDa Observed MW: 47 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Alpha-Enolase (ENO1)

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