EP300 (pS89) Antibody

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Target	EP300 (pS89)
Clonality	Polyclonal
Target Modification	Ser89
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, IHC, DB
Host	Rabbit
Recommended dilutions	IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q09472 (UniProt, ExPASy)
UniProt Secondary AC	B1AKC2
UniProt Entry Name	EP300_HUMAN
NCBI Accession	NP_001420.2
KEGG	hsa:2033
String	9606.ENSP00000263253
Molecular Weight	Calculated MW: 264 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse EP300.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on EP300 (pS89)

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