ER Alpha (ESR1) Antibody

The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.

Target	ER Alpha (ESR1)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. coli.
Isotype	IgG1
Form	Liquid
Purification	Purified from ascites by Protein G chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P03372 (UniProt, ExPASy)
UniProt Secondary AC	Q13511, Q14276, Q5T5H7, Q6MZQ9, Q9NU51, Q9UDZ7, Q9UIS7
UniProt Entry Name	ESR1_HUMAN
Gene Symbol	ESR1
GeneID	2099
OMIM	133430
HGNC	3467
KEGG	hsa:2099
Ensembl	ENSG00000091831
String	9606.ENSP00000405330
Molecular Weight	68 kDa
Buffer	PBS, containing 0.03% sodium azide.
Concentration	1 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on ER Alpha (ESR1)

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